+34 630 961 713 info@cloverbiosoft.com

Application areas

Clinical & Biomedical Research

Build models for strain typing, response to treatment, biomarker discovery, and more using both MALDI-TOF and FTIR.

Food & Beverage

Classify raw materials, detect spoilage patterns, compare batches, or verify origin using spectral fingerprints.

Pharma & Biotech

Support QC, formulation consistency, and process monitoring with automated spectral analysis and anomaly detection.

Industrial & Material Science

Characterise polymers, coatings, and composites; group materials by spectra; and detect deviations from expected patterns.

Environmental & Agriculture

Analyse soil, water, or plant samples using MALDI/FTIR signatures combined with Machine Learning.

References

1. Automated web-based typing of Clostridiodes difficile ribotypes via MALDI-TOF MS. Mario Blázquez-Sánchez, Alejandro Guerrero-López, Ana Candela, Albert Belenger-Llorens, José Miguel Moreno, Carlos Sevilla-Salcedo, María Sánchez-Cueto, Manuel J. Arroyo, Mark Gutiérrez-Pareja, Vanessa Gómez-Verdejo, Pablo M. Olmos, Luis Mancera, Patricia Muñoz, Mercedes Marín, Lluis Alcalá, David Rodríguez-Temporal, Belén Rodríguez-Sánchez. BMC Bioinformatics, 2025, 26:181.

2. Characterization of a nosocomial outbreak caused by VIM-1 Klebsiella michiganensis using Fourier-Transform Infrared (FT-IR) spectroscopy. David Rodriguez-Temporal, María Sánchez Cueto, Sergio Buenestado-Serrano, Mario Blázquez-Sánchez, Emilia Cercenado, Mark Gutiérrez-Pareja, Andrea Molero-Salinas, Elena López-Camacho, Patricia Muñoz, David García De Viedma, Laura Pérez-Lago, Belén Rodríguez-Sánchez. The Journal of Hospital Infections, 2025, 162:16.

3. Analysis of high-molecular-weight proteins using MALDI-TOF MS and machine learning for the differentiation of clinically relevant Clostridioides difficile ribotypes. Ana Candela, David Rodriguez-Temporal, Mario Blázquez-Sánchez, Manuel J. Arroyo, Mercedes Marín, Luis Alcalá, Germán Bou, Belén Rodríguez-Sánchez & Marina Oviaño. European Journal of Clinical Microbiology & Infectious Diseases, Dec 2024.

4. Multicenter evaluation of Fourier transform infrared (FTIR) spectroscopy as a first-line typing tool for carbapenemase-producing Klebsiella pneumoniae in clinical settings. Ana Candela, David Rodríguez-Temporal, Pilar Lumbreras, Paula Guijarro-Sánchez, Manuel J. Arroyo, Fernando Vázquez, Alejandro Beceiro, Germán Bou, Patricia Muñoz, Marina Oviaño, Javier Fernández, Belen Rodriguez-Sánchez. Journal of Clinical Microbiology, November 2024.

5. Neisseria leonii sp. nov., isolated from the nose, lung, and liver of rabbits. Martin Boutroux, Sandrine Favre-Rochex, Olivier Gorgette, Gérald Touak, Estelle Mühle, Christiane Bouchier, Olivier Chesneau, Frédéric J. Veyrier, Dominique Clermont and Praveen Rahi. International Journal of systematic and evolutionary microbiology, July 2024.

6. Development and validation of a quick, automated and reproducible ATR-FTIR spectroscopy machine learning model for Klebsiella pneumoniae typing. Ângela Novais, Ana Beatriz Gonçalves, Teresa Ribeiro, Ana Freitas, Gema Méndez, Luis Mancera, Antonia Read, Valquiria Alves, Lorena López-Cerero, Jesús Rodríguez-Baño, Alvaro Pascual, and Luísa Peixe. Journal of Clinical Microbiology, Accepted. Dec 2023. (+info)

7. Rapid Discrimination of Pseudomonas aeruginosaST175 Isolates Involved in a Nosocomial Outbreak Using MALDI-TOF Mass Spectrometry and FTIR Spectroscopy Coupled with Machine Learning. Ana Candela, Manuel Arroyo, María Sánchez-Cueto, Mercedes Marín, Emilia Cercenado, Gema Méndez, Patricia Muñoz, Luis Mancera, David Rodriguez-Temporal and Belén Rodríguez-Sánchez. Transboundary and Emerging Diseases, Sep. 2023. (+info)

8. Discrimination between hypervirulent and non-hypervirulent ribotypes of Clostridioides difficileby MALDI-TOF mass spectrometry and machine learning. Ahmed Mohamed Mostafa Abdrabou, Issa Sy, Markus Bischoff, Manuel J. Arroyo, Sören L. Becker, Alexander Mellmann, Lutz von Müller, Barbara Gärtner, Fabian K. Berger. European Journal of Clinical, Sep 2023. (+info)

9. Direct detection of carbapenemase-producing Klebsiella pneumoniaeby MALDI-TOF analysis of full spectra applying machine learning. Eva Gato, Manuel J. Arroyo, Gema Méndez, Ana Candela, Bruno K Rodiño-Janeiro, Javier Fernández, Belen Rodriguez, Luis Mancera, Jorge Arca-Suárez, Alejandro Beceiro, Germán Bou, and Marina Oviaño. Journal of Clinical Microbiology, May 2023. (+info)

10. Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Molecular Typing of Acinetobacter baumanniiin Comparison with Orthogonal Methods. Eloise J. Busby, Ronan M. Doyle, Clara Leboreiro Babe, Kathryn A. Harris, Damien Mack, Gema Méndez-Cervantes, Denise M. O’Sullivan, Vicky Pang, Zahra Sadouki, Priya Solanki, Jim F. Huggett, Timothy D. McHugh, Emmanuel Q. Wey. Journal of Clinical Microbiology, May 2023. (+info)

11. Automatic discrimination of species within the Enterobacter cloacae complex using MALDI-TOF Mass Spectrometry and supervised algorithms. Candela, A. Guerrero-Lopez, M. Mateos, A. Gomez Asenjo, M.J. Arroyo, M. Hernández-García, R. del Campo, E. Cercenado, A. Cuenod, G. Méndez, L. Mancera, J. de Dios Caballero, L. Martínez-García, D. Gijón, M.I. Morosini, P. Ruiz-Garbajosa, A. Egli, R. Cantón, P. Muñoz, D. Rodriguez-Temporal, B. Rodriguez-Sanchez. Journal of Clinical Microbiolog, Feb. 2023. (+info)

12. Identification of Mycobacterium abscessussubspecies by MALDI-TOF Mass Spectrometry and Machine Learning.  Rodríguez-Temporal, L. Herrera, F. Alcaide, D. Domingo, G. Héry-Arnaud, J. van Ingen, A. Van den Bossche, A. Ingebretsen, C. Beauruelle, E. Terschlüsen, S. Boarbi, N. Vila-Olmo, M.J. Arroyo, G. Méndez, P. Muñoz, L. Mancera, M.J. Ruiz-Serrano, B. Rodríguez-Sánchez. Journal of Clinical Microbiology, Jan. 2023. (+info)

13. Ultrasensitive and rapid identification of ESRI developer- and piperacillin/tazobactam-resistant Escherichia coliby the MALDIpiptaz tests.  Rodríguez-Villodres, L. Gálvez-Benítez, M.J. Arroyo, G. Méndez, L. Mancera, A. Vila-Domínguez, J.A. Lepe-Jiménez, Y. Smani. Emerging Microbes & Infections, Aug. 2022. (+info)

14. Detection of azole resistance in Aspergillus fumigatus complex isolates using MALDI-TOF mass spectrometry. E. Zvezdanova, M.J. Arroyo, G. Méndez, A. Candela, L. Mancera, J. García, J. Lozano, R. Jiménez, I. Lozano, C. Castro, C. López, P. Muñoz, J. Guinea, P. Escribano. Clinical Microbiology and Infection, Feb. 2022 (+ info).

15. Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The future of Networking Data Analyisis With Online Software. Gato E, Anantharajah A, Arroyo MJ, Artacho MJ, Caballero JdD, Candela A, Chudějová K, Constanso IP, Elías C, Fernández J, Jiménez J, Lumbreras P, Méndez G, Mulet X, Pérez-Palacios P, Rodríguez-Sánchez B, Cantón R, Hrabák J, Mancera L, Martínez-Martínez L, Oliver A, Pascual Á, Verroken A, Bou G and Oviaño M. Frontiers in Microbiology, Jan. 2022. (+ info).

16.- Rapid and reproducible MALDI-TOF-based method for the detection of Vancomycin-resistant Enterococcus faeciumusing classifying algorithms.  Candela, M.J. Arroyo, A. Sánchez-Molleda, G. Méndez, L. Quiroga, A. Ruiz, E. Cercenado, M. Marín, P. Muñoz, L. Mancera, D. Rodríguez-Temporal, B. Rodríguez-Sánchez. MDPI. Diagnostic Microbiology and Infectious Disease, Jan. 2022. (+info)

17. Occurrence of the p019 Gene in the blaKPC-Harboring Plasmids: Adverse Clinical Impact for Direct Tracking of KPC-Producing Klebsiella pneumoniae by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry. E. Gato, I. P. Constanso, B. Kotska Rodiño-Janeiro, P. Guijarro-Sánchez, T. Alioto, M.J. Arroyo, G. Méndez, L. Mancera, M. Gut, I. Gut, M. Álvarez.-Tejado, G. Bou, M. Oviaño. Journal of Clinical Microbiology, Jul. 2021. (+info)

18. An Improved Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Data Analysis Pipeline for the Identification of Carbapenemase-Producing Klebsiella pneumoniae. E. Gato, I. P. Constanso, A. Candela, F. Galán, B. K. Rodiño-Janeiro, M. J. Arroyo, G. Méndez, L. Mancera, T. Alioto, M. Gut, I. Gut, M. Álvarez-Tejado, B. Rodríguez-Sánchez, G. Bou, M. Oviaño. Journal of Clinical Microbiology, Jun. 2021.. (+info)

19. Implementation of MALDI-TOF Mass Spectrometry and Peak Analysis: Application to the Discrimination of Cryptococcus neoformans Species Complex and Their Interspecies Hybrids. M.E. Zvezdanova, M.J. Arroyo, G. Méndez, J.V. Guinea Ortega, L. Mancera, P. Muñoz, B. Rodríguez-Sánchez, P. Escribano. Journal of Fungi, Dec. 2020.

20. MALDI-MS Protein Profiling of Chemoresistance in Extracellular Vesicles of Cancer Cells. G. Stübiger, M.D. Nairn, T.K. Abban, M.E. Openshaw, L. Mancera, B. Herzig, M. Wuczkowski, D. Senfter, R.M. Mader. Analytical Chemistry, Nov. 2018.

21. Characterization of Yeasts and Filamentous Fungi using MALDI Lipid Phenotyping. G. Stübiger, M.D. Nairn, T.K. Abban, M.E. Openshaw, L. Mancera, B. Herzig, M. Wuczkowski, D. Senfter, R.M. Mader. Journal of Microbiological Methods, Nov. 2016.